Simultaneous Imaging of Intracellular DNA and RNA Using Harmless Light

Early detection of cellular damage that leads to aging or death is essential for developing therapeutic strategies for many diseases. Achieving this requires observing cellular changes throughout their life cycle by cell imaging. However, current methods cannot capture early responses or distinguish multiple injury states, especially in ultraviolet-visible (UV-vis)–sensitive cells.　These limitations can result in delayed diagnoses, an incomplete understanding of cellular fate after treatment, and misinterpreted therapeutic effects.　Therefore, there is a need for a universal, highly sensitive imaging method that uses harmless infrared to near-infrared excitation light to safely monitor complete cell states.

The research team successfully visualized both DNA and RNA inside living cells safely and simultaneously by using two types of harmless excitation light and fluorescent dye probes (N-heteroacene dyes) that bind differently to DNA and RNA. In addition to assessing sustained DNA damage through DNA imaging, the study revealed that RNA imaging provides higher sensitivity for predicting early stages of cell damage and aging. This dual DNA/RNA imaging enables early evaluation of cellular damage and precise detection of all four stages of cell death. The approach surpasses the limitations of current imaging systems by enabling visualization of single-cell state transitions.
It opens new possibilities for ultra-early detection of cellular damage and aging, non-toxic live-cell diagnostics, and high-throughput drug screening workflows.

The team plans to apply this method to living organisms in future studies. They aim to establish techniques for early disease detection, cellular stress monitoring, and precision medical strategies.　Ultimately, they hope to develop technologies that can determine a “pre-disease” state—when a person is drifting away from health—just by observing their cells.

• This study was conducted by Linawati Sutrisno (ICYS Research Fellow, NIMS), Gary J. Richards (Postdoctoral Researcher, MANA, NIMS), Michio Matsumoto (Researcher, Molecules Group, MANA, NIMS), Jonathan P. Hill (Group Leader, Functional Chromophores Group, MANA, NIMS), and Katsuhiko Ariga (Specially Appointed Researcher, MANA, NIMS); Koichiro Uto (Principal Researcher, Smart Polymer Group, Research Center for Macromolecules and Biomaterials, NIMS); Xianglan Li (NIMS Engineer, Bioanalysis Unit, Research Network and Facility Services Division); Jack D. Evans (Researcher, University of Adelaide); Masayasu Taki (Professor, Gifu University); and Shigehiro Yamaguchi (Professor, Nagoya University).
• The findings were published online in the open-access journal Science Advances on October 23, 2025.