Press Release 2012
Safe and efficient solid-mediated transfection by silica film composed by upright-sheet network
Potential cell therapy for hemophilia, diabetes or intractable diseases and genetic disorders
Researchers in MANA Supermolecules Unit and Biomaterials Unit have successfully developed a new silica film composed by upright-sheets network for the substrate-mediated reverse gene transfection. The dense upright nanosheet network favors the immobilizaiton of DNA and subsequent cell transfection even without recombinant proteins. Transgene expression indicated that the silica film possesses higher transfection efficiency than the conventional solution-based transfection. Due to not requiring the animal originated gene transfer agents in this case, a more simple, safe and effective gene transfection is expected.
Figure 1. TEM image of upright-sheet silica film
Figure 2. Gene transfection by upright-sheet silica film
Further information
Affiliation
Qingmin Ji1*, Tomohiko Yamazaki1, Nobutaka Hanagata2*, Michael V. Lee2, Jonathan P. Hill1, 3, Katsuhiko Ariga*1, 3
- 1.Institute for Materials Science (NIMS)
- 2.Interdisciplinary Laboratory for Nanoscale Science and Technology (INST), NIMS
- 3.JST, CREST
Contact information
About material preparation
Email: Katsuhiko Ariga, <ARIGA.Katsuhiko
nims.go.jp>
Telephone: +81-29-860-4597
Email: Qingmin Ji, <JI.Qingminnims.go.jp>
Telephone: +81-29-860-3354 (ext. 8923)
nims.go.jp>Telephone: +81-29-860-4597
Email: Qingmin Ji, <JI.Qingmin
nims.go.jp>Telephone: +81-29-860-3354 (ext. 8923)
About gene transfection
Email: Tomohiko Yamazaki, <YAMAZAKI.Tomohikonims.go.jp>
Telephone: +81-29-860-4845
Email: Nobutaka Hanagata, <HANAGATA.Nobutakanims.go.jp>
Telephone: +81-29-860-4774
nims.go.jp>Telephone: +81-29-860-4845
Email: Nobutaka Hanagata, <HANAGATA.Nobutaka
nims.go.jp>Telephone: +81-29-860-4774