Research Topics 2012

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Safe and efficient solid-mediated transfection by silica film composed by upright-sheet network

Potential cell therapy for hemophilia, diabetes or intractable diseases and genetic disorders

August 2, 2012

Researchers in MANA Supermolecules Unit and Biomaterials Unit have successfully developed a new silica film composed by upright-sheets network for the substrate-mediated reverse gene transfection. The dense upright nanosheet network favors the immobilizaiton of DNA and subsequent cell transfection even without recombinant proteins. Transgene expression indicated that the silica film possesses higher transfection efficiency than the conventional solution-based transfection. Due to not requiring the animal originated gene transfer agents in this case, a more simple, safe and effective gene transfection is expected.

Fig.1

Figure 1. TEM image of upright-sheet silica film

Fig.1

Figure 2. Gene transfection by upright-sheet silica film

Further information

Publications and Affiliation:

Qingmin Ji1*, Tomohiko Yamazaki1, Nobutaka Hanagata2*, Michael V. Lee2, Jonathan P. Hill1, 3, Katsuhiko Ariga*1, 3, Silica-based gene reverse transfection: an upright nanosheet network for promoted DNA delivery to cells, Chem. Commun., 2012, 48, 8496-8498.

  1. Supermolecures Unit, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS)
  2. Interdisciplinary Laboratory for Nanoscale Science and Technology (INST), NIMS
  3. JST, CREST

* corresponding authors

Contact information

About material preparation

Email: Katsuhiko Ariga, <ARIGA.Katsuhikonims.go.jp>
Telephone: +81-29-860-4597

Email: Qingmin Ji, <JI.Qingminnims.go.jp>
Telephone: +81-29-860-3354 (ext. 8923)

About gene transfection

Email: Tomohiko Yamazaki, <YAMAZAKI.Tomohikonims.go.jp>
Telephone: +81-29-860-4845

Email: Nobutaka Hanagata, <HANAGATA.Nobutakanims.go.jp>
Telephone: +81-29-860-4774

About MANA

http://www.nims.go.jp/mana/

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