研究活動

・J. Saito, X. Deng, A. Okamoto, “Single-Cell Mass Spectroscopic Analysis for Quantifying Active Metabolic Pathway Heterogeneity in a Bacterial Population on an Electrode”, Analytical chemistry, 92 23, 15616-15623 (2020)

・X. Deng, J. Saito, A. Kaksonen, A. Okamoto, “Enhancement of cell growth by uncoupling extracellular electron uptake and oxidative stress production in sediment sulfate-reducing bacteria”, Environment International, 144, 106006 (2020)

・M. Shiibashi, X. Deng, W. Miran, A. Okamoto, “Mechanism of Anaerobic Microbial Corrosion Suppression by Mild Negative Cathodic Polarization on Carbon Steel”, Environmental Science & Technology Letters 7, 690-694 (2020) 

・S. Hirano, J. Saito, T. Yukawa, D. Sano, A. Okamoto, S. Okabe, M. Kitajima, “Improvement of Electrochemical Conditions for Detecting Redox Reaction of K3 [Fe (CN) 6] toward the Application in Norovirus Aptasensor”, Electrochemistry, 88, 205-209 (2020)

・D. Naradasu, A. Guionet, T. Okinaga, T. Nishihara, A. Okamoto, “Electrochemical Characterization of Current Producing Human Oral-pathogens by Whole-Cell Electrochemistry”, ChemElectroChem, 7, 2012-2019 (2020) 

・E. La Cava, A. Guionet, J. Saito, A. Okamoto, “Involvement of Proton Transfer for Carbon Dioxide Reduction Coupled with Extracellular Electron Uptake in Shewanella oneidensis MR-1” Electroanalysis, 32 1659-1663 (2020)

・D. Naradasu, A. Guionet, W. Miran, A. Okamoto, “Microbial current production from Streptococcus mutans correlates with biofilm metabolic activity”, Biosensors and Bioelectronics, 112236 (2020) 

・D. Naradasu, W. Miran, A. Okamoto, “Metabolic Current Production by an Oral Biofilm Pathogen Corynebacterium matruchotii”, Molecules 25 (14), 3141 (2020)

・X. Deng, N. Dohmae, A. H. Kaksonen, A. Okamoto, “Biogenic Iron Sulfide Nanoparticles to Enable Extracellular Electron Uptake in Sulfate-Reducing Bacteria”, Angew. Chem. Int. Ed., 59, 5995-5999 (2020) Selected as VIP paper and Inside Cover 

・A. I. Garber, K. H. Nealson, A. Okamoto, S. M. McAllister, C. S. Chan, R. A. Barco, N. Merino, “FeGenie: A Comprehensive Tool for the Identification of Iron Genes and Iron Gene Neighborhoods in Genome and Metagenome Assemblies”, Front. Microbiol., 11, 37 (2020)

・S. Zhang, W. Miran, D. Naradasu, S. Guo, A. Okamoto, “A Human Pathogen Capnocytophaga Ochracea Exhibits Current Producing Capability”, Electrochemistry, 88, 224-229 (2020)

2019

・D. Naradasu, W. Miran, M. Sakamoto, A. Okamoto, “Isolation and Characterization of Human Gut Bacteria Capable of Extracellular Electron Transport by Electrochemical Techniques”, Front. Microbiol., 9, 3267 (2019)

・Y. Tokunou, A. Okamoto, “Geometrical Changes in the Hemes of Bacterial Surface c‐Type Cytochromes Reveal Flexibility in Their Binding Affinity with Minerals”, Langmuir, 35, 7529-7537 (2019)


2018

・X. Deng, N. Dohmae, K. H. Nealson, K. Hashimoto, A. Okamoto, “Multi-heme Cytochromes Provide a Pathway for Survival in Energy-limited Environments”, Sci. Adv., 4, eaao5682 (2018). 

・Y. Tokunou, P. Chinotaikul, S. Hattori, T. A. Clarke, L. Shi, K. Hashimoto, K. Ishii, A. Okamoto, “Whole-cell Circular Dichroism Difference Spectroscopy Reveals an in vivo-specific Deca-heme Conformation in Bacterial Surface Cytochromes”, Chemical Communications, 54, 13933-13936 (2018) 

・M. Murugan, W. Miran, T. Masuda, D. S. Lee, A. Okamoto, “Biosynthesized Iron Sulfide Nanocluster Enhanced Anodic Current Generation by Sulfate Reducing Bacteria in Microbial Fuel Cells”, ChemElectroChem, 5, 4015-4020 (2018).

・X. Deng, A. Okamoto, “Electrode Potential Dependency of Single-Cell Activity Identifies the Energetics of Slow Microbial Electron Uptake Process”, Front. Microbiol., 9, 2744 (2018). 

・A. Okamoto, A. R. Rowe, X. Deng, K. H. Nealson, “Self-Standing Electrochemical Set-up to Enrich Anode-respiring Bacteria On-Site”, J. Vis. Exp., 137, e57632 (2018).

・Y. Tokunou, K. Hashimoto, A. Okamoto, “Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1”, J. Vis. Exp., 134, e57584 (2018).

・A. R. Rowe, P. Rajeev, A. Jain, S. Pirbadian, A. Okamoto, J. A. Gralnick, M. Y. El-Naggar, K. H. Nealson, “Tracking Electron Uptake from a Cathode into Shewanella Cells: Implications for Energy Acquisition from Solid-Substrate Electron Donors”, mBio, 9, e02203-17 (2018).


2017

・J. Saito, K. Hashimoto, A. Okamoto, “Nanoscale Secondary Ion Mass Spectrometry Analysis of Individual Bacterial Cells Reveals Feedback from Extracellular Electron Transport to Upstream Reactions”, Electrochemistry, 85, 444-446 (2017).

・A. Okamoto, Y. Tokunou, S. Kalathil, K. Hashimoto, “Proton Transport in the Outer-Membrane Flavocytochrome Complex Limits the Rate of Extracellular Electron Transport”, Angew. Chem. Int. Ed., 56, 9082-9086 (2017).

・A. R. Rowe, M. Yoshimura, D. E. LaRowe, L. J. Bird, J. P. Amend, K. Hashimoto, K. H. Nealson, A. Okamoto, “in situ Electrochemical Enrichment and Isolation of a Magnetite-reducing Bacterium from a High pH Serpentinizing Spring”, Environ. Microbiol., 19, 2272-2285 (2017).


2016

・J. Saito, K. Hashimoto, A. Okamoto, “Flavin as an Indicator of the Rate-Limiting Factor for Microbial Current Production in Shewanella oneidensis MR-1”, Electrochim. Acta, 216, 261-265 (2016).
・Y. Tokunou, K. Hashimoto, A. Okamoto, “Acceleration of Extracellular Electron Transfer by Alternative Redox-Active Molecules to Riboflavin for Outer-Membrane Cytochrome c of Shewanella oneidensis MR-1”, J. Phys. Chem. C, 120, 16168-16173 (2016).


2015

・K. Kondo, A. Okamoto, K. Hashimoto, R. Nakamura, “Sulfur-Mediated Electron S.ttling Sustains Microbial Long-Distance Extracellular Electron Transfer with the Aid of Metallic Iron Sulfides”, Langmuir, 31, 7427-7434 (2015). 

・X. Deng, R. Nakamura, K. Hashimoto, A. Okamoto, “Electron Extraction from an Extracellular Electrode by Desulfovibrio ferrophilus Strain IS5 without using Hydrogen as an Electron Carrier”, Electrochemistry, 83, 529-531 (2015). 

・A. R. Rowe, P. Chellamuthu, B. Lam, A. Okamoto, K. H. Nealson, “Marine Sediment Microbes Capable of Electrode Oxidation as a Surrogate for Lithotrophic Insoluble Substrate Metabolism”, Front. Microbiol., 5, 784 (2015). 

・Y. Tokunou, K. Hashimoto, A. Okamoto, “Extracellular Electron Transport Scarcely Accumulates Proton Motive Force in Shewanella oneidensis MR-1”, Bull. Chem. Soc. Jap., 88, 690-692 (2015). 


2014

・A. Okamoto, K. Saito, K. Inoue, K. H. Nealson, K. Hashimoto, R. Nakamura, “Uptake of Self-secreted Flavins as Bound Cofactors for Extracellular Electron Transfer in Geobacter species”, Energy and Environmental Science, 7, 1357-1361 (2014). Selected as HOT article

・S. Kalathil, K. Hashimoto, A. Okamoto, “Effect of Ionic Strength on the Rate of Extracellular Electron Transport in Shewanella oneidensis MR-1 through Bound-Flavin Semiquinones”, ChemElectroChem, 53, 10988-10991 (2014). 

・A. Okamoto, S. Kalathil, X. Deng, K. Hashimoto, R. Nakamura, K. H. Nealson, “Cell-secreted Flavins Bound to Membrane Cytochromes Dictate Electron Transfer Reactions to Surfaces with Diverse Charge and pH”, Sci. Rep., 4, 5628 (2014). 

・A. Okamoto, K. Hashimoto, K. H. Nealson, “Flavin Redox Bifurcation as a Mechanism for Controlling the Direction of Electron Flow during Extracellular Electron Transfer”, Angew. Chem. Int. Ed., 53, 10988-10991 (2014).


2013-2007

・A. Okamoto, K. Hashimoto, K. H. Nealson, R. Nakamura, “Rate Enhancement of Bacterial Extracellular Electron Transport Involves Bound Flavin Semiquinones”, Proc. Natl. Acad. Sci, USA, 110, 7856-7861 (2013). 

・R. Nakamura, F. Kai, A. Okamoto, K. Hashimoto, “Mechanisms of Long-distance Extracellular Electron Transfer of Metal-reducing Bacteria Mediated by Nanocolloidal Semiconductive Iron Oxides”, J. Mater. Chem., 1, 5148-5157 (2013).

・A. Okamoto, R. Nakamura, K. Hashimoto, “Long-range Electron Conduction of Shewanella Biofilms Mediated by Outer Membrane C-type Cytochromes”, Bioelectrochemistry, 85, 61-65 (2012).

・A. Okamoto, R. Nakamura, K. Hashimoto, “in vivo Identification of Direct Electron Transfer from Shewanella oneidensis MR-1 to Electrodes via Outer Membrane OmcA-MtrCAB Protein Complex”, Electrochimica Acta, 56, 5526-5531 (2011). 

・R. Nakamura, A. Okamoto, N. Tajima, G. J. Newton, F. Kai, T. Takashima, K. Hashimoto, “Biological Iron-Monosulfide Production for Efficient Electricity Harvesting from a Deep-Sea Metal-Reducing Bacterium”, ChemBioChem, 11, 643-645 (2010). 

・A. Okamoto, R. Nakamura, K. Ishii, K. Hashimoto, “in vivo Electrochemistry of C Type Cytochrome-Mediated Electron-Transfer with Chemical Marking”, ChemBioChem, 10, 2329-2332 (2009). 

・R. Nakamura, F. Kai, A. Okamoto, G. J. Newton, K. Hashimoto, “Self-constructed Electrically Conductive Bacterial Networks”, Angew. Chem. Int. Ed., 48, 508-511 (2009). (IF値　12.959)

・A. Okamoto, R. Nakamura, H. Osawa, K. Hashimoto, “Anchored Oxo-Bridged Bimetallic Complexes, (SiO)3-Ti-O-Fe(corrole), on Silica Mesopores as Multi-Electron-Transfer Photosystems”, J. Phys. Chem. C, 112, 19777-19783 (2008). 

・A. Okamoto, R. Nakamura, H. Osawa, K. Hashimoto, “Site-specific Synthesis of Oxo-bridged Mixed-valence Binuclear Complexes on Mesoporous Silica”, Langmuir, 24, 7011-7017 (2008). 

・R. Nakamura, A. Okamoto, H. Osawa, H. Irie, K. Hashimoto, “Design of All-Inorganic Molecular-based Photocatalysts Sensitive to Visible Light: Ti(IV)-O-Ce(III) Bimetallic Assemblies on Mesoporous Silica”, J. Am. Chem. Soc., 129, 3084-3085 (2007).