Publication | Mechanobiology group

Ultimately Adaptive Fluid Interfacial Phospholipid Membranes Unveiled Unanticipated High Cellular Mechanical Work

Zhou Lu, Mizuki Tenjimbayashi, Junhong Zhou, and Jun Nakanishi*, Zhou Lu, Mizuki Tenjimbayashi, Junhong Zhou, and Jun Nakanishi*, Adv. Mater. in press. DOI: 10.1002/adma.202403396

Ionic Liquid Interface as a Cell Scaffold

Takeshi Ueki, Koichiro Uto, Shota Yamamoto, Ryota Tamate, Yuji Kamiyama, Xiaofang Jia, Hidenori Noguchi, Kosuke Minami, Katsuhiko Ariga, Hongxin Wang, Jun Nakanishi, Adv. Mater., 2310105 (2024). DOI: 10.1002/adma.202310105

Photoactivatable surfaces resolve the impact of gravity vector on collective cell migratory characteristics

Shinya Sakakibara, Shimaa A. Abdellatef, Shota Yamamoto, Masao Kamimura, Jun Nakanishi, Sci. Technol. Adv. Mater., 24: 2206525 (2023). DOI: 10.1080/14686996.2023.2206525

Mapping stress inside living cells by atomic force microscopy in response to environmental stimuli

Hongxin Wang, Han Zhang, Ryo Tamura, Bo Da, Shimaa A. Abdellatef, Ikumu Watanabe, Nobuyuki Ishida, Daisuke Fujita, Nobutaka Hanagata, Tomoki Nakagawa, Jun Nakanishi, Sci. Technol. Adv. Mater., 24, 2265434 (2023). DOI: 10.1080/14686996.2023.2265434

Relationship between CD4+ T-cell counts at baseline and initial periodontal treatment efficacy in patients undergoing treatment for HIV infection: A retrospective observational study

Tomoaki Shintani, Miho Okada, Tomoyuki Iwata, Maiko Kawagoe, Naoya Yamasaki, Tomoko Inoue, Jun Nakanishi, Daisuke Furutama, Katsuhiro Takeda, Toshinori Ando, Miyuki Nakaoka, Noriyoshi Mizuno, Teruhisa Fujii, Mikihito Kajiya, Hideki Shiba, Journal of Clinical Periodontology, 50, 1520–1529 (2023). DOI: 10.1111/jcpe.138730

Manipulating the Dynamic Adaptivity of a Fluid Interface to Maintain the Multipotency of Mesenchymal Stromal Cells

Wenyan Lyu, Wei Hu, Jiaming Shi, Jieman Chen, Jingwen Song, Qindan Zhang, Xuefeng Yuan, Dairui Li, Jun Nakanishi, Xiaofang Jia, Adv. Healthcare Mater., 12, 2300666 (2023).DOI: 10.1002/adhm.202300666

Growth and Migration Blocking Effect of Nanaomycin K, a Compound Produced by Streptomyces sp., on Prostate Cancer Cell Lines In Vitro and In Vivo

Hirata, Y.; Shigemura, K.; Moriwaki, M.; Iwatsuki, M.; Kan, Y.; Ooya, T.; Maeda, K.; Yang, Y.; Nakashima, T.; Matsuo, H.; et al. Growth and Migration Blocking Effect of Nanaomycin K, Cancers, 15, 2684 (2023). DOI: 10.3390/cancers15102684

Polarity Does Not Matter: Molecular Weight Reverses the Photoisomerization‐Induced Phase Separation of an Azobenzene‐Bearing Polymer

Kenta Homma, Alice C Chang, Shota Yamamoto, Takeshi Ueki, Jun Nakanishi, Macromol. Rapid Commun., 44, 2300118 (2023). DOI: 10.1002/marc.202300118

MP20-19 ANTI-TUMOR EFFECT OF NANAOMYCIN K, A COMPOUND EXTRACTED FROM STREPTOMYCES, ON PROSTATE CANCER CELL LINES IN VITRO AND IN VIVO

Yuto Hirata, Katsumi Shigemura, Michika Moriwaki, Masato Iwatsuki, Tooru Ooya, Yuki Kan, Maeda Koki, Youngmin Yang, Takuji Nakashima, Hirotaka Matsuo, Jun Nakanishi, and Masato Fujisawa, The Journal of Urology, 209, e283 (2023). DOI: 10.1097/JU.0000000000003245.19

Mechanistic investigation into selective cytotoxic activities of gold nanoparticles functionalized with epidermal growth factor variants

Aiwen Zhang, Shimaa A Abdellatef, Jun Nakanishi*, Anal. Sci., 39, 395–405 (2023), DOI: 10.1007/s44211-022-00256-7

Static and photoresponsive dynamic materials to dissect physical regulation of cellular functions

A. Zhang, S. A. Abdellatef, and J. Nakanishi*, Anal. Sci. 39: 395 (2023). DOI: 10.1007/s44211-022-00256-7

Highly stretchable and self-healable polymer gels from physical entanglements of ultrahigh–molecular weight polymers

Yuji Kamiyama, Ryota Tamate , Takashi Hiroi, Sadaki Samitsu, Kenta Fujii, Takeshi Ueki, Sci. Adv. 8, eadd0226 (2022). DOI:10.1126/sciadv.add0226

Adaptive liquid interfaces induce neuronal differentiation of mesenchymal stem cells through lipid raft assembly

Xiaofang Jia, Jingwen Song, Wenyan Lv, Jonathan P Hill, Jun Nakanishi, Katsuhiko Ariga, Nat Comm, 13: 1-10 (2022), DOI: 10.1038/s41467-022-30622-y

Precise Tuning and Characterization of Viscoelastic Interfaces for the Study of Early Epithelial–Mesenchymal Transition Behaviors

Alice Chinghsuan Chang, Koichiro Uto, Shimaa A Abdellatef, Jun Nakanishi, Langmuir, 38: , 5307–5314 (2022), DOI: 10.1021/acs.langmuir.1c03048

Geometrical and Mechanical Nanoarchitectonics at Interfaces Bridging Molecules with Cell Phenotypes

Jun Nakanishi, Shota Yamamoto, System-Materials Nanoarchitectonics, 275–286 (2022), 10.1007/978-4-431-56912-1_16

Design of azobenzene-bearing hydrogel with photoswitchable mechanics driven by photo-induced phase transition for in vitro disease modeling

Kenta Homma, Alice C Chang, Shota Yamamoto, Ryota Tamate, Takeshi Ueki, Jun Nakanishi, Acta Biomaterialia, 132: 103-113 (2021), DOI: 10.1016/j.actbio.2021.03.028

Dynamic Interfaces for Mechanobiological Studies”, in Material-based Mechanobiology

J. Nakanishi and K. Tatematsu, J. Nakanishi and K. Uto Eds., RSC (2022). DOI: 10.1039/9781839165375-00110.

An Introduction to Material-based Mechanobiology”, in Material-based Mechanobiology

J. Nakanishi and K. Uto, J. Nakanishi and K. Uto Eds., RSC (2022). DOI: 10.1039/9781839165375-00001.

Material-based Mechanobiology

J. Nakanishi and K. Uto, RSC (2022). eISBN: 978-1-83916-537-5.

Design of azobenzene-bearing hydrogel with photoswitchable mechanics driven by photo-induced phase transition for in vitro disease modeling

Kenta Homma, Alice C. Chang, Shota Yamamoto, Ryota Tamate, Takeshi Ueki, Jun Nakanishi, Acta Biomaterialia, 132, 103-113 (2021). DOI: 10.1016/j.actbio.2021.03.028.

Trends in biomaterials in Japan

Jun Nakanishi, Mitsuhiro Ebara, Sci. Technol. Adv. Mater., 22: 808 (2021), DOI: 10.1080/14686996.2021.1969097

Improved anti-cancer effect of epidermal growth factor-gold nanoparticle conjugates by protein orientation through site-specific mutagenesisImproved anti-cancer effect of epidermal growth factor-gold nanoparticle conjugates by protein orientation through site-specific mutagenesis

Aiwen Zhang, Jun Nakanishi, Science and Technology of Advanced Materials, 22:616-626 (2021), DOI: 10.1080/14686996.2021.1944783

Viscoelastically tunable substrates elucidate the interface-relaxation-dependent adhesion and assembly behaviors of epithelial cells

Alice Chinghsuan Chang, Koichro Uto, Kenta Homma, Jun Nakanishi, Biomaterials, 274: 120861 (2021), DOI: 10.1016/j.biomaterials.2021.120861

Analytical Biomaterials

Takahito Oshiro, Jun Nakanishi, Tamotsu Zako, Mizuo Maeda, Takahito Ohshiro, Anal. Sci., 37: 649 (2021), DOI: 10.2116/analsci.GE2105

Large-Area Aligned Fullerene Nanocrystal Scaffolds as Culture Substrates for Enhancing Mesenchymal Stem Cell Self-Renewal and Multipotency

J. Song, X. Jia*, K. Minami, J. P. Hill, J. Nakanishi, L. K. Shrestha, and K. Ariga*, ACS Appl. Nano Mater., 3, 6497-6506 (2020). DOI: 10.1021/acsanm.0c00973.

Nanaomycin K inhibited epithelial mesenchymal transition and tumor growth in bladder cancer cells in vitro and in vivo

Koichi Kitagawa, Katsumi Shigemura, Aya Ishii, Takuji Nakashima, Hirotaka Matsuo, Yoko Takahashi, Satoshi Omura, Jun Nakanishi, Masato Fujisawa, Sci. Rep., 11: 1-10 (2020), DOI: 10.1038/s41598-021-88741-3

Adaptive Liquid Interfacially Assembled Protein Nanosheets for Guiding Mesenchymal Stem Cell Fate

X. Jia*, K. Minami, K. Uto, A. C. Chang, J. P. Hill, J. Nakanishi*, and K. Ariga*, Adv. Mater., 32: 1905942 (2020). DOI: 10.1002/adma.201905942

A facile assay of epithelial-mesenchymal transition based on cooperativity quantification of cellular autonomous motions

S. Yamamoto, T. Miyama, T. Komoda, M. Sugawara, M. Nonomura, and J. Nakanishi*, Anal. Sci., 36: 263-268 (2020). DOI: 10.2116/analsci.19P233.

Nanaomycin K, a new epithelial-mesenchymal transition inhibitor produced by the actinomycete “Streptomyces rosa subsp. notoensis

H. Matsuo, J. Nakanishi, Y. Noguchi, K. Kitagawa, K. Shigemura, T. Sunazuka, Y. Takahashi, S. Omura, T. Nakashima,* J. Biosci. Bioeng., 129: 291-295 (2020). DOI: 10.1016/j.jbiosc.2019.09.007.

Photoactivatable hydrogel interfaces for resolving the interplay of chemical, mechanical, and geometrical regulation of collective cell migration

S. Yamamoto, K. Okada, N. Sasaki, A. Chang, K. Yamaguchi, J. Nakanishi*, Langmuir, 35: 7459-7468 (2019). DOI: 10.1021/acs.langmuir.8b02371.

Preparation of a Series of Photoresponsive Polymersomes Bearing Photocleavable a 2-nitrobenzyl Group at the Hydrophobic/Hydrophilic Interfaces and Their Payload Releasing Behaviors

S. Yamamoto, T. Yamada, G. Kubo, K. Sakurai, K. Yamaguchi,* J. Nakanishi*, Polymers, 11: 1254 (2019). DOI: 10.3390/polym11081254.

Synthesis of Poly(N-vinylpyrrolidone)-Based Polymer Bottlebrushes by ATRPA and RAFT Polymerization: Toward Drug Delivery Application

Y.S. Huang, J.K. Chen, S.W. Kuo, Y.A. Hsieh, S. Yamamoto, J. Nakanishi,* C.F. Huang*, Polymers, 11(6): 1079 (2019). DOI: 10.3390/polym11061079.

Epidermal growth factor-nanoparticle conjugates change the activity from anti-apoptotic to pro-apoptotic at membrane rafts

S. Yamamoto, Y. Iwamaru, Y. Shimizu, Y. Ueda, M. Sato, K. Yamaguchi, J. Nakanishi*, Acta Biomaterialia, 88: 383-391 (2019). DOI: 10.1016/j.actbio.2019.02.026.

Modulation of Mesenchymal Stem Cells Mechanosensing at Fluid Interfaces by Tailored Self-Assembled Protein Monolayers

X. Jia,* K. Minami, K. Uto, A. C. Chang, J. P. Hill, T. Ueki, J. Nakanishi,* K. Ariga*, Small, 15: 1804640 (2019). DOI: 10.1002/smll.201804640.

Nanaomycin I and J: new nanaomycins generated by mycothiol-mediated compounds from “Streptomyces rosa subsp. notoensis” OS-3966

H. Matsuo, Y. Noguchi, A Také, J. Nakanishi, K. Shigemura, T. Sunazuka, Y. Takahashi, S. Ōmura, and T. Nakashima*, J. Biosci. Bioeng., 127: 549-553 (2019). DOI: 10.1016/j.jbiosc.2018.10.013.

An application of photoactivatable substrate for the evaluation of epithelial-mesenchymal transition inhibitors

J. Nakanishi,* K. Sugiyama, H. Matsuo, Y. Takahashi, S. Ōmura, and T. Nakashima, Anal. Sci., 35: 65-70 (2019). DOI: 10.2116/analsci.18SDP07.

Chapter 18: Mechanobiology

J. Nakanishi,* Materials Nanoarchitectonics, K. Ariga, M. Ebara Ed., Wiley (2018). DOI: 10.1002/9783527808311.ch18.

A dynamic biomaterial based on a 2-nitrobenzyl derivative with a tert-butyl substituent at the benzyl position: rapid response and minimized phototoxicity

S. Yamamoto, H. Ikegami, K. Yamaguchi,* and J. Nakanishi*, ChemPhotoChem, 2: 786-790 (2018). Selected as a Front Cover. DOI: 10.1002/cptc.201800087.

Photoactivatable substrates for systematic study of the impact of an extracellular matrix ligand on appearance of leader cells in collective cell migration

S. A. Abdellatef and J. Nakanishi,* Biomaterials, 169: 72-84 (2018). DOI: 10.1016/j.biomaterials.2018.03.045.

Suppression of Myogenic Differentiation of Mammalian Cells Caused by Fluidity of a Liquid-Liquid Interface

K. Minami, T. Mori, W. Nakanishi, N. Shigi, J. Nakanishi, J. P. Hill, M. Komiyama, and K. Ariga,* ACS Appl. Mater. Interf., 9: 30553-30560 (2017). DOI: 10.1021/acsami.7b11445.

Nanaomycin new analog, nanaomycin H

T. Nakashima,* T. Kimura, R. Miyano, H. Matsuo, T. Hirose, A. Kimishima, K. Nonaka, M. Iwatsuki, J. Nakanishi, Y. Takahashi, and S. Ōmura, J. Biosci. Bioeng., 123: 765-770 (2017). DOI:10.1016/j.jbiosc.2017.01.011.

Photoactivatable Substrates: A Material-based Approach for Dissecting Cell Migration

J. Nakanishi,* Chem. Rec., 17 (6): 611-621 (2017). DOI:10.1002/tcr.201600090.

Facile Preparation of Photoactivatable Surfaces with Tuned Substrate Adhesiveness

Y. Shimizu, M. Kamimura, S. Yamamoto, S. A. Abdellatef, K. Yamaguchi, and J. Nakanishi,* Anal. Sci., 32: 1183-1188 (2016). DOI:10.2116/analsci.32.1183.

Past, Present, and Future of Mechanobiology: from the Viewpoint of Biomaterial Researcher

J. Nakanishi,* M. Ebara, and C. Yoshikawa, J. Jpn. Soc. Biomater. (Japanese), 34 (2): 106-111 (2016).

What Are Emerging Concepts and Challenges in NANO?: Nanoarchitectonics, Hand-Operating Nanotechnology, and Mechanobiology

K. Ariga,* K. Minami, M. Ebara, and J. Nakanishi, Polymer J., 48, 371-389 (2016). DOI:10.1038/pj.2016.8.

Estradiol-tethered micropatterned surfaces for the study of estrogenic non-genomic pathways

B. Qi, Y. Shimizu, J. Nakanishi,*, and F. M. Winnik,* Chem. Commun., 52: 10056-10059 (2016). DOI:10.1039/C6CC03899A.

Methods for Measuring Forces in Cellular Mechanobiology

J. Nakanishi,* Buseki (Japanese), (1), 25-26, (2016).

Reduced Adhesive Ligand Density in Engineered Extracellular Matrices Induces an Epithelial-Mesenchymal-Like Transition

S. Marlar, S. A. Abdellatef, and J. Nakanishi*, Acta Biomaterialia, 39: 106-113 (2016). DOI:10.1016/j.actbio.2016.05.006.

3.3 – Cell Manipulation Technologies

J. Nakanishi,* Biomaterials Nanoarchitectonics, M. Ebara Ed. Elsevier (2016). DOI: 10.1016/B978-0-323-37127-8.00008-X.

Dynamic Control of Cell Adhesion on a Stiffness-Tunable Substrate for Analyzing the Mechanobiology of Collective Cell Migration

M. Kamimura, M. Sugawara, S. Yamamoto, K. Yamaguchi, and J. Nakanishi*, Biomater. Sci., 4: 933-937 (2016). Selected as an Outside Cover. DOI:10.1039/C6BM00100A.