Personal Information

Name: Hirokazu Komatsu (小松　広和)

Birth: Jan 1979, age 36

Gender: Male

Present Address: MANA Supermolecules Group

National Institute for Materials Science (NIMS)

E-mail: KOMATSU.Hirokazu@nims.go.jp

Language: Japanese, English

Professional Background: Analytical Chemistry, Photochemistry, Organic Chemistry

Research Field: Chemical Biology, Bio-analysis, Functional molecules

Interested in : Bioparallel chemistry, Artificial reaction promoters, Fluorescent probes

Education

2001.3 BS in Faculty of Science and Technology,

Keio University (Prof. Koji Suzuki)

2003.3 MS in Graduate School of Science and Technology,

Keio University (Prof. Koji Suzuki)

2006.3 Ph.D. in Graduate School of Science and Technology,

Keio University (Prof. Koji Suzuki)

Career

2003.4-2005.3 DC1 of the Japan Society for the Promotion of Sciences

2005.4-2007.3 Assistant

in Faculty of Science and Technology,

Keio University (Prof. Koji Suzuki)

2008.10-2009.12 Researcher

in ASTEM,

Kyoto University (Prof. Sei-ichi Nishimoto)

2010.1-2012.3 Researcher

in Graduate School of Engineering,

Kyoto University (Prof. Sei-ichi Nishimoto)

2012.4-2013.3 Researcher (ERATO project)

in Graduate School of Pharmaceutical,

Tokyo University (Prof. Motomu Kanai)

2013.4- Researcher

in Supermolecules Group, MANA-YAMATO program

National Institute for Materials Science(NIMS) (Prof.Katsuhiko Ariga)

Publication List (Total number of publication = 51, h-index=13 by Web of Science) 2015.3.16

Peer-Reviewed Articles (22)

1)Akamatsu, Masaaki; Taizo, Mori; Ken, Okamoto; Hirokazu, Komatsu; Ken, Kumagai; Seimei, Shiratori; Masaki, Yamamura; Tatsuya, Nabeshima; Hideki, Sakai; Masahiko, Abe; Hill, Jonathan P.; and Katsuhiko, Ariga

Detection of Ethanol in Alcoholic Beverages or Vapor Phase Using Fluorescent Molecules Embedded in a Nanofibrous Polymer

ACS Applied Materials & Interface published on web

DOI: 10.1021/acsami.5b00289

2) Komatsu, Hirokazu*; Daimon, Yuki; Kawakami, Kohsaku; Kanai, Motomu; Hill, Jonathan P.; Ariga, Katsuhiko*

Reaction Mediated Artificial Cell Termination: Control of Vesicle Viability using Rh(I)-Catalyzed Hydrogenation

Phys. Chem. Chem. Phys., 2014, 16(31), 16454-16457.

10.1039/C4CP02255F

3) Akamatsu, Masaaki; Komatsu, Hirokazu*; Mori, Taizo; Adams, Eri; Shin, Ryoung; Sakai, Hideki; Abe, Masahiko; Hill, Jonathan P.; Ariga, Katsuhiko*

Intracellular Imaging of Cesium Distribution in Arabidopsis Using Cesium Green

ACS Applied Materials & Interfaces (2014), 6(11), 8208-8211.

10.1021/am5009453

4) Komatsu, Hirokazu*; Shindo, Yutaka; Oka, Kotaro; Hill, Jonathan P.; Ariga, Katsuhiko*

Ubiquinone-Rhodol (UQ-Rh) for Fluorescence Imaging of NAD(P)H through Intracellular Activation

Angewandte Chemie, International Edition (2014), 53(15), 3993-3995.

10.1002/anie.201311192

5) Komatsu, Hirokazu; Shindo, Yutaka; Kawashima, Shigehiro A.; Yamatsugu, Kenzo; Oka, Kotaro; Kanai, Motomu

Intracellular activation of acetyl-CoA by an artificial reaction promoter and its fluorescent detection

Chemical Communications (2013), 49(28), 2876-2878.

10.1039/c3cc40616d

6)Komatsu, Hirokazu; Yoshihara, Kazuki; Yamada, Hisatsugu; Kimura, Yu; Son, Aoi; Nishimoto, Sei-ichi; Tanabe, Kazuhito

Ruthenium Complexes with Hydrophobic Ligands That Are Key Factors for the Optical Imaging of Physiological Hypoxia

Chemistry--A European Journal (2013), 19(6), 1971-1977.

10.1002/chem.201202809

7) Shindo, Yutaka; Fujii, Tomohiko; Komatsu, Hirokazu; Citterio, Daniel; Hotta, Kohji; Suzuki, Koji; Oka, Kotaro

Newly Developed Mg2+–Selective Fluorescent Probe Enables Visualization of Mg2+

Dynamics in Mitochondria.

PLoS ONE, (2011), 6(8): e23684.

10.1371/journal.pone.0023684

8)Komatsu, Hirokazu; Tanabe, Kazuhito; Nishimoto, Sei-ichi

¹³C-labeled indolequinone-DTPA-Gd conjugate for NMR probing cytochrome:P450 reductase-mediated one-electron reduction

Bioorg. Med. Chem. Lett., (2011), 21(2), 790-793.

10.1016/j.bmcl.2010.11.105

9)Komatsu, Hirokazu; Harada, Hiroshi; Tanabe, Kazuhito; Hiraoka, Masahiro; Nishimoto, Sei-ichi. Indolequinone-rhodol conjugate as a fluorescent probe for hypoxic cells: enzymatic activation and fluorescence properties

Med. Chem. Commun., (2010), 1, 50 - 53

10.1039/c0md00024h

10)Tanabe, Kazuhito; Harada, Hiroshi; Narazaki, Michiko; Tanaka, Kazuo; Inafuku, Kenichi; Komatsu, Hirokazu; Ito, Takeo; Yamada, Hisatsugu; Chujo, Yoshiki; Matsuda, Tetsuya; Hiraoka, Masahiro; Nishimoto, Sei-ichi. Monitoring of Biological One-Electron Reduction by ¹⁹F NMR Using Hypoxia Selective Activation of an ¹⁹F-Labeled Indolequinone Derivative

Journal of the American Chemical Society, (2009), 131 (44), 15982–15983.

10.1021/ja904953b

11) Kojima, Mikiko; Kamada-Nobusada, Tomoe; Komatsu, Hirokazu; Takei, Kentaro; Kuroha, Takeshi; Mizutani, Masaharu; Ashikari, Motoyuki; Ueguchi-Tanaka, Miyako; Matsuoka, Makoto; Suzuki, Koji; Sakakibara, Hitoshi.

Highly sensitive and high-throughput analysis of plant hormones using MS-probe modification and liquid chromatography-tandem mass spectrometry: an application for hormone profiling in Oryza sativa

Plant and Cell Physiology (2009), 50(7), 1201-1214.

10.1093/pcp/pcp057

12) Suzuki, Miho; Husimi, Yuzuru; Komatsu, Hirokazu; Suzuki, Koji; Douglas, Kenneth T.

Quantum Dot FRET Biosensors that Respond to pH, to Proteolytic or Nucleolytic Cleavage, to DNA Synthesis, or to a Multiplexing Combination. Journal of the American Chemical Society (2008), 130(17), 5720-5725.

10.1021/ja710870e

13) Honda, Aki; Komatsu, Hirokazu; Kato, Dai; Ueda, Akio; Maruyama, Kenichi; Iwasaki, Yuzuru; Ito, Takeshi; Niwa, Osamu; Suzuki, Koji.

Newly developed chemical probes and nano-devices for cellular analysis. Analytical Sciences (2008), 24(1), 55-66.

10.2116/analsci.24.55

14) Hifumi, Hiroki; Tanimoto, Akihiro; Citterio, Daniel; Komatsu, Hirokazu; Suzuki, Koji.

Novel 15-crown-5 ether or b -diketone incorporated gadolinium complexes for the detection of potassium ions or magnesium and calcium ions. Analyst (2007), 132(11), 1153-1160.

10.1039/b707225b

15) Citterio, Daniel; Takeda, Junichiro; Kosugi, Masaki; Hisamoto, Hideaki; Sasaki, Shin-ichi; Komatsu, Hirokazu; Suzuki, Koji.

pH-Independent Fluorescent Chemosensor for Highly Selective Lithium Ion Sensing. Analytical Chemistry (2007), 79(3), 1237-1242.

10.1021/ac061674g

16) Komatsu, Hirokazu; Miyachi, Makiko; Fujii, Eiji; Citterio, Daniel; Yamada, Koji; Sato, Yoshinobu; Kurihara, Kazuyoshi; Kawaguchi, Haruma; Suzuki, Koji.

SPR sensor signal amplification based on dye-doped polymer particles. Science and Technology of Advanced Materials (2006), 7(2), 150-155.

10.1016/j.stam.2005.12.009

17) Komatsu, Hirokazu; Miki, Takahiro; Citterio, Daniel; Kubota, Takeshi; Shindo, Yutaka; Kitamura, Yoshiichiro; Oka, Kotaro; Suzuki, Koji.

Single Molecular Multianalyte (Ca2+, Mg2+) Fluorescent Probe and Applications to Bioimaging. Journal of the American Chemical Society (2005), 127(31), 10798-10799.

10.1021/ja0528228

18) Komatsu, Hirokazu; Citterio, Daniel; Fujiwara, Yukio; Minamihashi, Katsuya; Araki, Yoshio; Hagiwara, Masafumi; Suzuki, Koji.

Single Molecular Multianalyte Sensor: Jewel Pendant Ligand. Organic Letters (2005), 7(14), 2857-2859.

10.1021/ol0507219

19) Kubota, Takeshi; Shindo, Yutaka; Tokuno, Kentaro; Komatsu, Hirokazu; Ogawa, Hiroto; Kudo, Susumu; Kitamura, Yoshiichiro; Suzuki, Koji; Oka, Kotaro.

Mitochondria are intracellular magnesium stores: investigation by simultaneous fluorescent imagings in PC12 cells. Biochimica et Biophysica Acta, Molecular Cell Research (2005), 1744(1), 19-28.

10.1016/j.bbamer.2004.10.013

20) Komatsu, Hirokazu; Iwasawa, Naoko; Citterio, Daniel; Suzuki, Yoshio; Kubota, Takeshi; Tokuno, Kentaro; Kitamura, Yoshiichiro; Oka, Kotaro; Suzuki, Koji.

Design and synthesis of highly sensitive and selective fluorescein-derived magnesium fluorescent probes and application to intracellular 3D Mg2+ imaging. Journal of the American Chemical Society (2004), 126(50), 16353-16360.

10.1021/ja049624l

21) Shoda, Takuji; Kikuchi, Kazuya; Kojima, Hirotatsu; Urano, Yasuteru; Komatsu, Hirokazu; Suzuki, Koji; Nagano, Tetsuo.

Development of selective, visible light-excitable, fluorescent magnesium ion probes with a novel fluorescence switching mechanism. Analyst (2003), 128(6), 719-723.

10.1039/b212835g

22) Suzuki, Yoshio; Komatsu, Hirokazu; Ikeda, Takafumi; Saito, Naohiko; Araki, Sawa; Citterio, Daniel; Hisamoto, Hideaki; Kitamura, Yoshiichiro; Kubota, Takeshi; Nakagawa, Jun; Oka, Kotaro; Suzuki, Koji.

Design and synthesis of Mg2+-selective fluoroionophores based on a coumarin derivative and application for Mg2+ measurement in a living cell. Analytical Chemistry (2002), 74(6), 1423-1428.

10.1021/ac010914j

Non-Reviewed Articles (4)

1)Tanabe. Katuhito; Hirokazu, Komatsu; Harada, Hiroshi; Hiraoka, Masahiro; Nishimoto, Sei-ichi.

Fluorescence imaging of hypoxic cells using indolequinone derivative activated by enzymatic one-electron reduction

Photomedicine and Photobiology (2010), 32, 41-42.

2)Tanabe, Kazuhito; Komatsu, Hirokazu; Harada, Hiroshi; Hiraoka, Masahiro; Nishimoto, Sei-ichi.

Monitoring of hypoxia-selective enzymatic reduction of indolequinone derivative by 19F-NMR.

Photomedicine and Photobiology (2009), 31, 31-32.

3)Komatsu, Hirokazu; Oka, Kotaro; Suzuki, Koji.

A single molecular multianalyte fluorescent probe and its application to imaging intracellular multianalyte dynamics.

American Laboratory (2006), 38(18), 24,26-29.

4)Yoshio, Suzuki; Naohiko Saito, Hirokazu Komatsu, Daniel Citterio, Yoshiichiro Kitamura, Takeshi Kubota, Kotaro Oka, Koji Suzuki

Design and Application of Novel Fluorescent Indicators of Mg2+ Based on Coumarin Derivative

Anal.Sci, (2002), 17, i1451-i1454.

Reviewed Proceedings of International Conference (9)

1)Komatsu, Hirokazu; Harada, Hiroshi; Yamada, Hisatsugu; Ito, Takeo; Tanabe, Kazuhito; Hiraoka, Masahiro; Nishimoto, Sei-ichi

Design of hypoxia-sensitive fluorescent probes activated by enzymatic one-electron reduction for hypoxic cancer cell imaging Pacifichem 2010, ANYL-941.

2) Shindo, Yutaka; Komatsu, Hirokazu; Hotta, Kohji; Suzuki, Koji; Oka, Ketaro.

Analysis of expression levels of Mg2+ permeable channels in cellular model of Parkinson's disease in PC12 cells NEUROSCIENCE RESEARCH, 61, S167-S167 (2008)

3) Makino, Mikiko; Ueda, Nanae; Takei, Kentaro; Komatsu, Hirokazu; Tanjii, Noriyuki; Suzuki, Koji; Sakakibara, Hitoshi.

High-throughput analysis of major plant hormones PLANT AND CELL PHYSIOLOGY (48), S199-S199 (2007)

4) Komatsu, Hirokazu; Shindo, Yutaka; Kubota, Takeshi; Hotta, Koji; Oka, Kotaro; Suzuki, Koji. Mitochondrial magnesium imaging. Abstracts of Papers, 232nd ACS National Meeting, San Francisco, CA, United States, Sept. 10-14, 2006 (2006), ANYL-330.

5) Komatsu, Hirokazu; Shindo, Yutaka; Kubota, Takeshi; Hotta, Koji; Oka, Kotaro; Suzuki, Koji. Rhodamine-based magnesium fluorescent probes for imaging of mitochondrial magnesium dynamics. Abstracts of Papers, 231st ACS National Meeting, Atlanta, GA, United States, March 26-30, 2006 (2006), ANYL-418.

6) T. Miki, H.Komatsu, N.Iwasawa, D. Citterio, T,Kubota, Y.shindo, Y.Kitamura, K.Oka, K.Suzuki. Design and synthesis of single molecular multianalyte fluorescent probes and their applications to bioimaging. Pacifichem 2005 Meeting, Honolulu, USA, 2005.

7) Komatsu, Hirokazu; Miki, Takahiro; Shindo, Yutaka; Kitamura, Yoshiichiro; Oka, Kotaro; Suzuki, Koji. Single molecular multi analyte fluorescent probes for bioimaging. Abstracts of Papers, 230th ACS National Meeting, Washington, DC, United States, Aug. 28-Sept. 1, 2005 (2005), ANYL-490.

8) Komatsu, Hirokazu; Miki, Takahiro; Fujiwara, Yukio; Citterio, Daniel; Kubota, Takeshi; Shindo, Yutaka; Kitamura, Yoshiichiro; Hagiwara, Masafumi; Oka, Kotaro; Suzuki, Koji. Single molecular multi analyte fluorescent probes and their intracellular app Komatsu, Hirokazu lications. Abstracts of Papers, 229th ACS National Meeting, San Diego, CA, United States, March 13-17, 2005 (2005), ANYL-178.

9) Kubota, Takeshi; Shindo, Yutaka; Tokuno, Kentaro; Komatsu, Hirokazu; Ogawa, Hiroto; Kitamura, Yoshiichiro; Suzuki, Koji; Oka, Kotaro. Investigation of intracellular magnesium mobilization pathways in Pc12 cells by simultaneous Mg-Ca fluorescent imaging. Journal of the American College of Nutrition (2004), 23(6), 742S-744S.

Accounts, Reviews, Books (11)

1) Komatsu, Hirokazu; Akamatsu, Masaaki; Ji, Qinmin; Hill, Jonathan P.; Ariga, Katsuhiko, (review), Displays and Imaging, accepted.

2) 有賀克彦, 小松広和 : “植物細胞内でのセシウム分布状態の可視化” Isotope News(日本アイソトープ協会広報誌) 730 (2015) 14-15

3) 有賀克彦、小松広和、中西和嘉、植物細胞内でのセシウムの分布の可視化

O plus E, 2014年11月、1201-1202.

4) 小松広和、森泰蔵、有賀克彦, 固体表面や生物中におけるセシウム分布状況を可視化する蛍光材料

放射線物質除去技術集成、エヌ・ティー・エス, 2014. (book)

5) Ariga, Katsuhiko; Komatsu Hirokazu; Jonathan P. Hill. Nanophotonics and Supramolecular Chemistry. Nanophotonics (2013), 2(4), 265-277.

10.1515/nanoph-2013-0025

6) 実験化学講座２０－１分析化学ｐ５６３～ｐ５７３（鈴木孝治、小松広和）

１２．３節、バイオイメージング用分子プローブ、丸善、平成１９年１月(book)

7) Shindo, Yutaka; Komatsu, Hirokazu; Oka, Kotaro; Suzuki, Koji. Development of fluorescent probes for intracellular magnesium measurement and their applications to analysis of cellular mechanisms. Tanpakushitsu Kakusan Koso (2007), 52(13, Zokan), 1552-1557.

8) Komatsu, Hirokazu; Suzuki, Koji. Molecular sensor based on chemistry of recognition. Kagaku (Kyoto, Japan) (2007), 62(1), 68-69.

9) Komatsu, Hirokazu; Oka, Kotaro; Suzuki, Koji. Development of Ca2+/Mg2+ multifluorescent probe for simultaneous multicomponent analysis. Seibutsu Kogaku Kaishi (2006), 84(5), 173-175.

10) Suzuki, Koji; Komatsu, Hirokazu; Kurihara, Kazuyoshi. Surface plasmon resonance sensor. Gendai Kagaku (2006), 418 17-22.

11) Komatsu, Hirokazu; Suzuki, Yoshio; Suzuki, Koji. Fluorescent probes for intracellular Mg2+ measurement. Clinical Calcium (2004), 14(8), 1200-1203.

Patent(5)

1.「蛍光プローブ、および、ニコチンアデニンジヌクレオチド誘導体を検出する方法」特願2013-006978

2.「細胞内アセチル化のイメージング試薬」特願2012-225841

3.「マグネシウムイオン及びカルシウムイオン同時測定用プローブ」特願2004-10433

4.「第二銅イオン測定用プローブ及びそれを用いた第二銅イオンの測定方法」特願2004-8475

5.「アルミニウムイオンおよび/又は第二鉄イオン測定用プローブ」特願2004-101389