Abstract Takayuki Uchihashi is a professor of Physics Department at Nagoya University and a visiting professor of Exploratory Research Center on Life and Living Systems, National Institute of Natural Science, Japan. He received his B. Sc., M.Sc. in Physics from Hiroshima University in 1993 and 1995, respectively. In 1998, he obtained Dc. Eng. in Electronics from Osaka University. From 1998 to 2000, he worked at Joint Research Center for Atom Technology (JRCAT) in Tsukuba as a research associate. In 2000, he moved to Department of Electronic Engineering, Himeji Institute of Technology as an assistant professor. After that he moved to Trinity College in Dublin in 2002 and worked as a senior researcher in SFI Nanoscience Institute. In 2004, he joined the Physics Department, Kanazawa University as an assistance professor. He became an associate professor in 2008, and a full professor in Physics Department in 2015. In 2017, he moved to Department of Physics, Nagoya University. His research interests include the instrumentation of scanning probe microscopy and its application to biological science.26Atomic Force Microscopy (AFM) has become an indispensable tool for visualizing biological molecules in liquid environments at nanometer resolution, enabling the observation of a diverse range of specimens from single molecules to cells. One of the most coveted capabilities of AFM was “high-speed imaging,” which held the promise of directly visualizing dynamic processes occurring at solid-liquid interfaces. This dream was realized in 2001 with the advent of high-speed AFM. Since its inception, capable of imaging biomolecules at 80 ms/frame, HS-AFM has overcome the limitations of conventional AFM, enabling the direct visualization of dynamic phenomena at solid-liquid interfaces. Over the past two decades, significant advancements in instrumentation, including reduced invasiveness and expanded functionalities, have propelled HS-AFM to the forefront of dynamic nanoscale investigations across diverse disciplines.Beyond its initial applications in visualizing biological molecules like proteins at nanometer resolution, HS-AFM has demonstrated remarkable versatility in investigating a wide range of dynamic behaviors in various systems, including synthetic supramolecules and polymers. By capturing real-time changes in these systems, HS-AFM provides crucial insights into the mechanisms underlying molecular interactions, assembly processes, and conformational dynamics. This has led to groundbreaking discoveries in dynamic structural biology and materials science, facilitating unprecedented observations of protein conformational changes, complex interactions, and self-assembly processes.This presentation will showcase the transformative power of HS-AFM by delving into several recent imaging applications. We will focus on the diverse types of dynamic phenomena observable with HS-AFM, encompassing conformational dynamics of biomolecules, protein-protein interactions, and the dynamic behavior of synthetic supramolecules. I will highlight how HS-AFM is not only deepening our understanding of fundamental biological processes but also paving the way for novel explorations and discoveries across a broad spectrum of scientific disciplines.Professor, Department of Physics, Nagoya University Invited Talk: S1-3Beyond the Static: Unveiling Single-Molecule Dynamics with High-Speed Atomic Force MicroscopyTakayuki Uchihashi
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