Jun Nakanishi

Jun Nakanishi
Analytical Chemistry, Biomaterials Science, Chemical Biology
Academic degree:
Ph.D. University of Tokyo (2001)
Recent publications
See NIMS Researchers DB

Education and Research history

2007 - Present MANA Independent Scientist, MANA, NIMS
2006 Senior Researcher, National Institute for Materials Science (NIMS)
2005 PRESTO Researcher, JST
2005 Research Associate, Waseda University
2002 Special Postdoctoral Researcher, RIKEN
2001 JSPS Research Fellow
2001 Ph.D. Department of Chemistry, University of Tokyo
1998 M.D. Department of Chemistry, University of Tokyo
1996 B.D. Department of Chemistry, University of Tokyo

Research Interests

Cell migration:

Cell migration plays critical roles in various physiological and pathological processes. He took advantage of photoactivatable cell culture substrates to confine cells within a given geometry and induce their migration by controlled photo-irradiation of the substrate. For example, he demonstrated collective characteristics of cells became enhanced by increasing cluster size and culture time. This approach is useful not only for the systematic understanding of cell migration, but also for engineering migration characteristics for tissue engineering applications.

Dynamic cell micropatterning on a photoactivatable cell culture substrate:

Cell micropatterning is useful for tissue engineering, fundamental cellular studies, and cell alignment on sensing devices. He developed a glass coverslip modified with a silane-coupling agent having a photocleavable group, and applied it to dynamic cell micropatterning. In contrast to conventional methods, dynamic cell micropatterning allows one to in situ alteration of cell micropattern during cultivation. Based on this feature, he succeeded in guidance of protrusions from single cells and co-culturing of heterotypic single cells.

Fluorescence imaging of protein conformational change in living cells:

Proteins involved in intracellular signaling dynamically change their conformation upon activation. He synthesized fluorescent molecules that can be selectively attached to a peptide sequence in living cells. By genetically engineering a protein of interest to have the peptide sequence and labeled it with the fluorescent molecule, he developed a method for real-time imaging of protein conformational change in living cells. Based on this method, he succeeded in visualizing agonist-induced conformational dynamics of a cytosolic protein (calmodulin) and a membrane protein (β2 adrenergic receptor) in living cells.

Selected Papers

  1. Switchable adhesive substrates: Revealing geometry dependence in collective cell behavior
    C. G. Rolli, H. Nakayama, K. Yamaguchi, J. P Spatz, R. Kemkemer, and J. Nakanishi
    Biomaterials, 33, 2409-2418 (2012), Selected as a leading opinion paper
  2. Spatiotemporally controlled navigation of neurite outgrowth in sequential steps on the dynamically photo-patternable surface
    Y. Edagawa, J. Nakanishi, K. Yamaguchi, and N. Takeda
    Coll. Surf. B, 99, 20-26 (2012)
  3. Photocontrol of cell adhesion on amino-bearing surfaces by reversible conjugation of poly(ethylene glycol) via a photocleavable linker
    S. Kaneko, H. Nakayama, Y. Yoshino, D. Fushimi, K. Yamaguchi, Y. Horiike and J. Nakanishi
    Physical Chemistry Chemical Physics, 13, 4051-4059 (2011)
  4. Silane-coupling agent bearing a photoremovable succinimidyl carbonate for patterning amines on glass and silicon surfaces with controlled surface densities
    H. Nakayama, J. Nakanishi, T. Shimizu, Y. Yoshino, H. Iwai, S. Kaneko, Y. Horiike and K. Yamaguchi
    Colloids and Surfaces B, 76 (1), 88-97 (2010)
  5. Light-Regulated Activation of Cellular Signaling by Gold Nanoparticles That Capture and Release Amines
    J. Nakanishi, H.Nakayama, T. Shimizu, H. Ishida, Y. Kikuchi, K. Yamaguchi, and Y. Horiike
    J. Am. Chem. Soc., 131, 3822-3823 (2009)
  6. Recent Advances in Cell Micropatterning Techniques for Bioanalytical and Biomedical Sciences
    J. Nakanishi, T. Takarada, K. Yamaguchi, and M. Maeda,
    Analytical Sciences, 24, 67-72 (2008), recieved "Most Cited Paper Award of Analytical Sciences -2010"
  7. Arraying Heterotypic Single Cells on Photoactivatable Cell-Culturing Substrates
    Y. Kikuchi, J. Nakanishi, H. Nakayama, S. Inoue, K. Yamaguchi,H. Iwai, Y. Yoshida, Y. Horiike, T. Takarada, and M. Maeda
    Langmuir, 24, 13084-13095 (2008)
  8. Grafting Poly(ethylene glycol) to a Glass Surface via a Photocleavable Linker for Light-induced Cell Micropatterning and Cell Proliferation Control
    Y. Kikuchi, J. Nakanishi, H. Nakayama, T. Shimizu, Y. Yoshino, K. Yamaguchi, Y. Yasuhiko, and Y. Horiike
    Chemistry Letters, 37, 1062-1063 (2008)
  9. Spatiotemporal Control of Migration of Single Cells on a Photoactivatable Cell-Microarray
    J. Nakanishi, Y. Kikuchi, S. Inoue, K. Yamaguchi, T. Takarada, and M. Maeda
    J. Am. Chem. Soc., 129, 6694-6695 (2007)
  10. FRET-based monitoring of conformational change of the β2 adrenergic receptor in living cells
    J. Nakanishi, T. Takarada, S. Yunoki, Y. Kikuchi, and M. Maeda
    Biochem. Biophys. Res. Commun., 343, 1191-1196 (2006)