Jun Nakanishi
- Affiliation:
- MANA, NIMS
- Specialty:
- Analytical Chemistry, Biomaterials Science, Chemical Biology
- Academic degree:
- Ph.D. University of Tokyo (2001)
- Recent publications
- See NIMS Researchers DB
Education and Research history
| 2007 | - | Present | MANA Independent Scientist, MANA, NIMS |
| 2006 | Senior Researcher, National Institute for Materials Science (NIMS) | ||
| 2005 | PRESTO Researcher, JST | ||
| 2005 | Research Associate, Waseda University | ||
| 2002 | Special Postdoctoral Researcher, RIKEN | ||
| 2001 | JSPS Research Fellow | ||
| 2001 | Ph.D. Department of Chemistry, University of Tokyo | ||
| 1998 | M.D. Department of Chemistry, University of Tokyo | ||
| 1996 | B.D. Department of Chemistry, University of Tokyo |
Research Interests
Cell migration:
Cell migration plays critical roles in various physiological and pathological processes. He took advantage of photoactivatable cell culture substrates to confine cells within a given geometry and induce their migration by controlled photo-irradiation of the substrate. For example, he demonstrated collective characteristics of cells became enhanced by increasing cluster size and culture time. This approach is useful not only for the systematic understanding of cell migration, but also for engineering migration characteristics for tissue engineering applications.
Dynamic cell micropatterning on a photoactivatable cell culture substrate:
Cell micropatterning is useful for tissue engineering, fundamental cellular studies, and cell alignment on sensing devices. He developed a glass coverslip modified with a silane-coupling agent having a photocleavable group, and applied it to dynamic cell micropatterning. In contrast to conventional methods, dynamic cell micropatterning allows one to in situ alteration of cell micropattern during cultivation. Based on this feature, he succeeded in guidance of protrusions from single cells and co-culturing of heterotypic single cells.
Fluorescence imaging of protein conformational change in living cells:
Proteins involved in intracellular signaling dynamically change their conformation upon activation. He synthesized fluorescent molecules that can be selectively attached to a peptide sequence in living cells. By genetically engineering a protein of interest to have the peptide sequence and labeled it with the fluorescent molecule, he developed a method for real-time imaging of protein conformational change in living cells. Based on this method, he succeeded in visualizing agonist-induced conformational dynamics of a cytosolic protein (calmodulin) and a membrane protein (β2 adrenergic receptor) in living cells.
Related Links
Award- Most Cited Paper Award of Analytical Sciences 2010, Jun. 21, 2011
- Young Scientist's Prize for the Commendation of Science and Technology by the Minister of Education, Culture, Sports, Science and Technology (MEXT), Apr. 11, 2011
- Japan Society for Analytical Chemistry Award for Younger Researchers, Sep. 25, 2009
Selected Papers
- Switchable adhesive substrates: Revealing geometry dependence in collective cell behavior
- C. G. Rolli, H. Nakayama, K. Yamaguchi, J. P Spatz, R. Kemkemer, and J. Nakanishi
- Biomaterials, 33, 2409-2418 (2012), Selected as a leading opinion paper
- Spatiotemporally controlled navigation of neurite outgrowth in sequential steps on the dynamically photo-patternable surface
- Y. Edagawa, J. Nakanishi, K. Yamaguchi, and N. Takeda
- Coll. Surf. B, 99, 20-26 (2012)
- Photocontrol of cell adhesion on amino-bearing surfaces by reversible conjugation of poly(ethylene glycol) via a photocleavable linker
- S. Kaneko, H. Nakayama, Y. Yoshino, D. Fushimi, K. Yamaguchi, Y. Horiike and J. Nakanishi
- Physical Chemistry Chemical Physics, 13, 4051-4059 (2011)
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- Silane-coupling agent bearing a photoremovable succinimidyl carbonate for patterning amines on glass and silicon surfaces with controlled surface densities
- H. Nakayama, J. Nakanishi, T. Shimizu, Y. Yoshino, H. Iwai, S. Kaneko, Y. Horiike and K. Yamaguchi
- Colloids and Surfaces B, 76 (1), 88-97 (2010)
- Light-Regulated Activation of Cellular Signaling by Gold Nanoparticles That Capture and Release Amines
- J. Nakanishi, H.Nakayama, T. Shimizu, H. Ishida, Y. Kikuchi, K. Yamaguchi, and Y. Horiike
- J. Am. Chem. Soc., 131, 3822-3823 (2009)
- Recent Advances in Cell Micropatterning Techniques for Bioanalytical and Biomedical Sciences
- J. Nakanishi, T. Takarada, K. Yamaguchi, and M. Maeda,
- Analytical Sciences, 24, 67-72 (2008), recieved "Most Cited Paper Award of Analytical Sciences -2010"
- Arraying Heterotypic Single Cells on Photoactivatable Cell-Culturing Substrates
- Y. Kikuchi, J. Nakanishi, H. Nakayama, S. Inoue, K. Yamaguchi,H. Iwai, Y. Yoshida, Y. Horiike, T. Takarada, and M. Maeda
- Langmuir, 24, 13084-13095 (2008)
- Grafting Poly(ethylene glycol) to a Glass Surface via a Photocleavable Linker for Light-induced Cell Micropatterning and Cell Proliferation Control
- Y. Kikuchi, J. Nakanishi, H. Nakayama, T. Shimizu, Y. Yoshino, K. Yamaguchi, Y. Yasuhiko, and Y. Horiike
- Chemistry Letters, 37, 1062-1063 (2008)
- Spatiotemporal Control of Migration of Single Cells on a Photoactivatable Cell-Microarray
- J. Nakanishi, Y. Kikuchi, S. Inoue, K. Yamaguchi, T. Takarada, and M. Maeda
- J. Am. Chem. Soc., 129, 6694-6695 (2007)
- FRET-based monitoring of conformational change of the β2 adrenergic receptor in living cells
- J. Nakanishi, T. Takarada, S. Yunoki, Y. Kikuchi, and M. Maeda
- Biochem. Biophys. Res. Commun., 343, 1191-1196 (2006)