Mechanobiology is a scientific field that studies the principle in sensing and generation of physical force in life. Recent studies in this field shed light on the significance of force, comparable to chemical and biochemical cues, in the regulation of biological processes. However, one unique feature in the mechanical regulation, distinct from chemical/biochemical one, is that it can directly react with the multi-layered architectures of living systems, ranging from nano-scale proteins, subcellular organelles, cells, tissues, organs to whole bodies. "International symposium on nanoarchitectonics for mechanobiology" stands at this viewpoint with a special attention to researches on novel molecules and materials to dissect and control mechano-nanoarchitectonics of life.